On Friday, Aria and I set up the germination chamber trial.
Method
- Count each seed quantity and label the edge of petri dishes with a variety/replication code, using tape and sharpie. 4 repetitions for each variety, total of 20 petri dishes.
- Place a filter paper piece in the bottom of each petri dish. Each filter paper should cover the bottom surface area of the dish. With a pencil, label 1-20 for each seed.
- Dampen each filter paper by pouring deionized H20 in each petri dish. Swirl each dish so water is evenly coated and let sit to absorb for a few seconds, Pour out any excess standing water by gently tipping the dish.
- Place 20 samples of seeds in the designated petri dish, making sure there is no overlapping.
- Every third day, moisten specimens with DI water as needed & record data.
- Record BBCH code data for all 20 seeds in each sample. Afterward, count and record frequency per code.
- When radicle is present, measure radicle length in mm – from the initial protrusion on the seed to the tip of the root.
- Take a photo and/or draw each code stage.
I formatted BBCH Germination Data Collection Sheets using Excel.
These are intended to be printed and used for real-time data collection. While the following excel document is intended for synthesizing data in excel.
Yield Data
