{"id":93,"date":"2024-05-26T03:05:54","date_gmt":"2024-05-26T03:05:54","guid":{"rendered":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/?p=93"},"modified":"2024-05-26T03:05:54","modified_gmt":"2024-05-26T03:05:54","slug":"week-6-reflection","status":"publish","type":"post","link":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/2024\/05\/26\/week-6-reflection\/","title":{"rendered":"Week 6 Reflection"},"content":{"rendered":"\n

FINALIZED METHOD FOR GERMINATION CHAMBER TRIAL<\/h2>\n\n\n\n
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http:\/\/ei.cornell.edu\/toxicology\/bioassays\/lettuce\/data.html<\/a><\/figcaption><\/figure><\/div>\n\n\n\n
  1. Count each seed quantity and label petri dishes using clear tape and sharpie. 20 seeds per petri dish. 4 repetitions for each variety. Total of 20 petri dishes.<\/li>
  2. Place equal sized filter paper pieces in the bottom of each petri dish. Each filter paper should cover the bottom surface area of each dish. <\/li>
  3. Dampen each filter paper by pouring 1 Tablespoon of deionized H2<\/sub>0 in each petri dish. Swirl each dish so water is evenly coated and let sit to absorb for a few seconds.<\/li>
  4. Evenly place each seed group(s) in trays, making sure there is no overlapping. <\/li>
  5. Moisten specimens with 1 T DI water per petri dish & record data every other day. <\/li>
  6. Record code data for seeds in each sample every other day. Code description will be marked per all 20 seeds. Afterward, average each petri dish sample codes as percentages. <\/li>
  7. When radicle is present, <\/em>measure radicle length in mm – from the initial protrusion on the seed to the tip of the root. <\/li>
  8. Take a photo and\/or draw each code stage. <\/li><\/ol>\n\n\n\n
    In our meeting this week, it's been decided that we'd like to begin our germination chamber study during week 8. We've established access to a growth chamber for 20 petri dishes with the generosity of faculty Robin Morgan, as well as filter paper and reused petri dishes in coordination with Diane Nelson at the SCC. Although, I hoped to begin this study sooner, defining the details and gaining tangible access is an ongoing process.      <\/pre>\n\n\n\n
    BBCH SCALE <\/h2>\n\n\n\n
    “The extended BBCH-scale is a system for a uniform coding of phenologically similar growth stages of all mono- and dicotyledonous plant species.
    It results from teamwork between the German Federal Biological Research Centre for Agriculture and Forestry (BBA), the German Federal Office of Plant Varieties (BSA), the German Agrochemical Association (IVA) and the Institute for Vegetables and Ornamentals in Grossbeeren\/Erfurt, Germany (IGZ). The decimal code, which is divided into principal and secondary growth stages, is based on the well-known cereal code developed by ZADOKS et al. (1974) in order to avoid major changes from this widely used phenological key.The abbreviation\u00a0BBCH\u00a0<\/strong>derives from\u00a0B<\/strong>iologische\u00a0B<\/strong>undesanstalt, Bundessortenamt and\u00a0CH<\/strong>emical industry.”<\/p>    Hack et al., 1992<\/a><\/cite><\/blockquote>\n\n\n\n
    Code<\/strong><\/td> Description<\/strong><\/td><\/tr>
    01<\/td> Beginning of seed imbibition<\/td><\/tr>
    03<\/td> Seed imbibition complete<\/td><\/tr>
    05<\/td> Radicle (root) emerged from seed<\/td><\/tr>
    06<\/td> Elongation of radicle, formation of root hairs and\/or lateral roots<\/td><\/tr>
    07<\/td> Hypocotyl with cotyledons or shoot breaking through seed coat<\/td><\/tr>
    08<\/td> Hypocotyl with cotyledon growth<\/td><\/tr>
    09<\/td> Hypocotyl with cotyledon growth through soil surface (not applicable to this study)<\/em><\/td><\/tr><\/tbody><\/table>
    Based on terminology derived from The extended BBCH-scale, general<\/strong>\u00a0(Hack et al., 1992<\/a>)<\/figcaption><\/figure>\n\n\n\n
    We will be focusing on the Principal Growth Stage 0: Germination<\/strong> (Federal Biological Research Centre for Agriculture and Forestry, 2001\u00a0(Hack et al., 1992<\/a>)). These specified stages and descriptors contribute to an effort towards defining a standardized language for measuring phenological habits of plant species. This is especially relevant to the ‘Gilo’ study, considering there is minimal phenological documentation and public databases on Solanum aetheopicum<\/em>. By using the BBCH scale, radicle length, and recording visual documentation; we hope to provide useful phenological and morphological data for growing Solanum aethiopicum<\/em>, ‘Gilo’, in the PNW climate. <\/p>\n\n\n\n
    BBCH Examples in Scientific Literature<\/h2>\n\n\n\n
    1. Acosta-Quezada, P.G., et al. \u201cPhenological growth stages of tree tomato (solanum betaceum CAV.), an emerging fruit crop, according to the basic and extended BBCH SCALES.\u201d\u00a0Scientia Horticulturae<\/em>, vol. 199, 16 Feb. 2016, pp. 216\u2013223, https:\/\/www.sciencedirect.com\/science\/article\/pii\/S030442381530368X<\/a><\/li>
    2. Hack, H., et al.\u00a0Growth Stages of Mono-and Dicotyledonous Plants, BBCH Monograph<\/em>, Federal Biological Research Centre for Agriculture and Forestry, 2001, www.politicheagricole.it\/flex\/AppData\/WebLive\/Agrometeo\/MIEPFY800\/BBCHengl2001.pdf.\u00a0<\/a><\/li><\/ol>\n","protected":false},"excerpt":{"rendered":"
      FINALIZED METHOD FOR GERMINATION CHAMBER TRIAL Count each seed quantity and label petri dishes using clear tape and sharpie. 20…<\/p>\n","protected":false},"author":787,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[1,6],"tags":[],"_links":{"self":[{"href":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/wp-json\/wp\/v2\/posts\/93"}],"collection":[{"href":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/wp-json\/wp\/v2\/users\/787"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/wp-json\/wp\/v2\/comments?post=93"}],"version-history":[{"count":5,"href":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/wp-json\/wp\/v2\/posts\/93\/revisions"}],"predecessor-version":[{"id":100,"href":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/wp-json\/wp\/v2\/posts\/93\/revisions\/100"}],"wp:attachment":[{"href":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/wp-json\/wp\/v2\/media?parent=93"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/wp-json\/wp\/v2\/categories?post=93"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/wp-json\/wp\/v2\/tags?post=93"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}