{"id":85,"date":"2024-05-10T15:19:26","date_gmt":"2024-05-10T15:19:26","guid":{"rendered":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/?p=85"},"modified":"2024-05-12T01:39:58","modified_gmt":"2024-05-12T01:39:58","slug":"week-5-reflection","status":"publish","type":"post","link":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/2024\/05\/10\/week-5-reflection\/","title":{"rendered":"Week 5 Reflection"},"content":{"rendered":"\n

Germination Chamber Parameters <\/h1>\n\n\n\n
Code<\/strong><\/td> Description <\/strong><\/td><\/tr>
01 <\/td>Beginning of seed imbibition <\/td><\/tr>
03<\/td>Seed imbibition complete<\/td><\/tr>
05<\/td>Radicle (root) emerged from seed<\/td><\/tr>
06<\/td>Elongation of radicle, formation of root hairs and\/or lateral roots<\/td><\/tr>
07 <\/td>Hypocotyl with cotyledons or shoot breaking through seed coat<\/td><\/tr>
08<\/td>Hypocotyl with cotyledon growth<\/td><\/tr>
09<\/td>Hypocotyl with cotyledon growth through soil surface (not applicable to this study)<\/em><\/td><\/tr><\/tbody><\/table>
Based on terminology derived from The extended BBCH-scale, general<\/strong> <\/a>(<\/a>Hack et al., 1992<\/a>)<\/figcaption><\/figure>\n\n\n\n

Method <\/strong><\/p>\n\n\n\n

  1. Count each seed quantity and label petri dishes using clear tape and sharpie. 15\/20(TBD) seeds per petri dish. 4 repetitions for each variety. Total of 20 petri dishes.<\/li>
  2. Place equal sized filter paper pieces in the bottom of each petri dish. Each filter paper should cover the bottom surface area of each dish. <\/li>
  3. Dampen each filter paper by pouring 1 Tablespoon of deionized H2<\/sub>0 in each petri dish. Swirl each dish so water is evenly coated and let sit to absorb for a few seconds.<\/li>
  4. Evenly place each seed group(s) in trays, making sure there is no overlapping. <\/li>
  5. Moisten specimens with 1 T DI water per petri dish & record data every other day.
    1. Record code data for each sample every other day. Code description will be marked per seed and as percentages % for each seed sample. <\/li>
    2. When radicle is present, <\/em>measure radicle length in mm – from the initial protrusion on the seed to the tip of the root. <\/li><\/ol><\/li><\/ol>\n\n\n\n

      Germination Chamber Data Collection <\/h2>\n\n\n\n
      • Code for each petri dish sample. A code will be collected based on results pertaining to 50% of each petri sample.<\/li>
      • Emergence count for each petri sample.
        • Code 07: Hypocotyl with cotyledons or shoot breaking through seed coat.<\/li><\/ul><\/li>
        • If present<\/em> Radicle length (mm) of each petri sample.<\/li><\/ul>\n","protected":false},"excerpt":{"rendered":"

          Germination Chamber Parameters Code Description 01 Beginning of seed imbibition 03 Seed imbibition complete 05 Radicle (root) emerged from seed…<\/p>\n","protected":false},"author":787,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[1,6],"tags":[],"_links":{"self":[{"href":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/wp-json\/wp\/v2\/posts\/85"}],"collection":[{"href":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/wp-json\/wp\/v2\/users\/787"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/wp-json\/wp\/v2\/comments?post=85"}],"version-history":[{"count":4,"href":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/wp-json\/wp\/v2\/posts\/85\/revisions"}],"predecessor-version":[{"id":91,"href":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/wp-json\/wp\/v2\/posts\/85\/revisions\/91"}],"wp:attachment":[{"href":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/wp-json\/wp\/v2\/media?parent=85"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/wp-json\/wp\/v2\/categories?post=85"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wordpress.evergreen.edu\/laurareusche2024gilovarietytrial\/wp-json\/wp\/v2\/tags?post=85"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}